GST-SUMO Activating Enzyme (E1)
- 貨號 Cat. No.
- E-310
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
GST-SUMO Activating Enzyme (E1)
S. cerevisiae
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.
140 kDa
> 90% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Catalog #: E-310
GST-SUMO Activating Enzyme (E1)
Product Source:
S. cerevisiae
Also know as: GST-SUMO Activating Enzyme (Aos1/Uba2)
Conjugation of the ubiquitin-like modifier SUMO requires the activities of the heterodimeric E1 (Aos1/Uba2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1 (also SUMO-2 and SUMO-3), forming a high-energy thiolester bond with the cysteine residue of Uba2 and the release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO-1 is transferred to Cys93 of UbcH9.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH8.0, 150 mM NaCl. Actual concentration will vary with specific Lot #.
MW:
140 kDa
Purity:
> 90% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.