GST- SUMO-1
- 貨號 Cat. No.
- UL-710
- 規格 Size
- 500 μg
- 品牌 Brand
- Boston Biochem
Description
GST- SUMO-1
human recombinant
Product Information
500 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
38.6 kDa
> 95% by SDS-PAGE
Use & Storage
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-710
GST- SUMO-1
Product Source:
human recombinant
Also known as: GST- SUMO-1 (SMT3C)
The ubiquitin-like SUMO-1 is conjugated to a variety of proteins in the presence of UbcH9 and the SAE1/SAE2 (human) or Aos1/Uba2 (yeast) activating enzyme. SUMO-1 is derived from the precursor pro-SUMO-1 (Accession # NM_003352). Human SUMO-1 shares 46% and 47% identity with SUMO-2 and SUMO-3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation.
Product Information
Quantity:
500 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 1mM DTT. Actual concentration varies with lot number.
MW:
38.6 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical in vitro concentrations for conjugate formation is 10-50 μM depending on conditions.
Storage:
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.