GST-NEDD8 Activating Enzyme (E1)
- 貨號 Cat. No.
- E-312
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
GST-NEDD8 Activating Enzyme (E1)
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl. Actual concentration will vary with specific lot number.
135 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Store at –80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-312
GST-NEDD8 Activating Enzyme (E1)
Product Source:
human recombinant
Also know as: GST-NEDD8 Activating Enzyme (APPBP1/Uba3)
The ATP-coupled activation of NEDD8 that is required for subsequent charging of the NEDD8-specific E2 UbcH12 is catalyzed by heterodimeric APPBP1-Uba3 in humans. The enzyme catalyzes the activation of the C-terminal carboxyl group of NEDD8 by forming a high-energy thioester bond in an ATP-dependent manner. Uba3 shows 43% homology to the C-terminal half of the ubiquitin activating E1 enzyme Uba1. The Uba3- catalyzed activation of NEDD8 exhibits an absolute requirement for APPBP1 which has high homology to the N-terminal half of Uba1.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl. Actual concentration will vary with specific lot number.
MW:
135 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Storage:
Store at –80°C. Avoid multiple freeze/thaw cycles.