GST-ISG15 Activating Enzyme (E1)
- 貨號 Cat. No.
- E-308
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
GST-ISG15 Activating Enzyme (E1)
human reconbinant
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number
138 kDa
> 95% by SDS-PAGE
Use & Storage
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Store at –80°C. Avoid multiple freeze/thaw cycles.
Catalog #: E-308
GST-ISG15 Activating Enzyme (E1)
Product Source:
human reconbinant
Also known as: UBA7
This E1 is responsible for the first step in ISG15-protein isopeptide bond formation. The enzyme catalyzes the activation of the C-terminal carboxyl group of ISG15 by forming a high-energy thioester bond in an ATP-dependent manner. The activated ISG15 is then transferred to UbcH8 and subsequently to lysine groups of target proteins via this thiolester conjugation cascade. The E1 enzyme is a critical component for the initiation of any in vitro conjugation reactions.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 8.0. Actual concentration varies with lot number
MW:
138 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Typical enzyme concentration to support conjugation in vitro is 50-200 nM depending on conditions.
Storage:
Store at –80°C. Avoid multiple freeze/thaw cycles.