GRK2 Polyclonal Ab
- 貨號 Cat. No.
- 3394-100
- 規格 Size
- 100 ug
Description
pimg src=http://www.biovision.com/Merchant2/graphics/00000001/antibody-stock-miva.jpg alt= /nbsp;/p
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pbFORMULATION: /b100 ug (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide./p
pbSPECIES REACTIVITY: /bhuman, mouse, rat/p
pbSTORAGE CONDITIONS: /bStore at -20oC. For long term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles./p
pbBACKGROUND: /bGRK2 (G-protein-coupled receptor kinase 2, also known as beta-ARK1) is a member of the GRK family that phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR. GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin and calmodulin, as well as by phosphorylation. PKC phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin. PKA phosphorylates GRK2 at serine685, which facilitates the association of GRK2 with a beta-adrenergic receptor. Erk inhibits GRK2 activity via phosphorylation at serine 670 of GRK2. Src phosphorylates GRK2 at multiple tyrosine residues (tyrosines 13, 86 and 92) that activates GRK2 activity and promotes GRK2 degradation./p
pbSPECIFICITY: /bThe antibody recognizes 80 kDa GRK2 of human, mouse, and rat origins./p
pbAPPLICATION: /bThe antibody has been used in Western blotting (1-2 ug/ml). However, the optimal concentrations should be determined individually. USAGE:/p
pbFor Research Use Only. Not to be used in human. /b/p
pfont size=2 /font/p
pbFORMULATION: /b100 ug (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide./p
pbSPECIES REACTIVITY: /bhuman, mouse, rat/p
pbSTORAGE CONDITIONS: /bStore at -20oC. For long term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles./p
pbBACKGROUND: /bGRK2 (G-protein-coupled receptor kinase 2, also known as beta-ARK1) is a member of the GRK family that phosphorylates the activated form of G-protein-coupled receptors (GPCRs) and initiates the desensitization process of GPCR. GRK2 kinase activity and cellular localization are tightly regulated by interactions with activated receptors, G-beta and G-gamma subunits, adaptor proteins, phospholipids, caveolin and calmodulin, as well as by phosphorylation. PKC phosphorylation enhances GRK2 activity by promoting its membrane localization and by abolishing the inhibitory association of calmodulin. PKA phosphorylates GRK2 at serine685, which facilitates the association of GRK2 with a beta-adrenergic receptor. Erk inhibits GRK2 activity via phosphorylation at serine 670 of GRK2. Src phosphorylates GRK2 at multiple tyrosine residues (tyrosines 13, 86 and 92) that activates GRK2 activity and promotes GRK2 degradation./p
pbSPECIFICITY: /bThe antibody recognizes 80 kDa GRK2 of human, mouse, and rat origins./p
pbAPPLICATION: /bThe antibody has been used in Western blotting (1-2 ug/ml). However, the optimal concentrations should be determined individually. USAGE:/p
pbFor Research Use Only. Not to be used in human. /b/p