GRB2 Polyclonal Ab
- 貨號 Cat. No.
- 3395-100
- 規格 Size
- 100 ul
Description
pimg src=http://www.biovision.com/Merchant2/graphics/00000001/antibody-stock-miva.jpg alt= //p
pfont size=2 /font/p
pbHOST:/b Rabbit/p
pbIMMUNOGEN:/b Synthetic peptide corresponding to C-terminus of human GRB2/p
pbFORMULATION: /b100 ug (0.5 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal./p
pbSPECIES REACTIVITY: /bhuman, mouse, rat/p
pbSTORAGE CONDITIONS: /bStore at -20oC. For long term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles./p
pbBACKGROUND: /bGRB2 (Growth factor receptor-binding protein 2) is an adaptor protein involving in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins such as EGFR, IRS-1, Shc and Gab1. The SH3 domain of GRB2 associates with Sos, which stimulates the GTP binding activity of Ras, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of tyrosine209 of GRB2 by Bcr-Abl and EGFR abolishes its association with Sos and negatively regulates downstream signaling./p
pbSPECIFICITY: /bThe antibody recognizes 25 kDa GRB2 from samples of human, mouse, and rat origins./p
pbAPPLICATION: /bThe antibody has been used in Western blotting (1 ug/ml). Based on customer?s feedback, the antibody can also be used in immunoprecipitation (10-20 ug/ml), and immunohistochemistry (15-25 ug/ml). However, the optimal concentrations should be determined individually. USAGE:/p
pbFor Research Use Only. Not to be used in human. /b/p
pfont size=2 /font/p
pbHOST:/b Rabbit/p
pbIMMUNOGEN:/b Synthetic peptide corresponding to C-terminus of human GRB2/p
pbFORMULATION: /b100 ug (0.5 mg/ml) immunoaffinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal./p
pbSPECIES REACTIVITY: /bhuman, mouse, rat/p
pbSTORAGE CONDITIONS: /bStore at -20oC. For long term storage, aliquot and freeze at -70oC. Avoid repeated freeze/defrost cycles./p
pbBACKGROUND: /bGRB2 (Growth factor receptor-binding protein 2) is an adaptor protein involving in RTK signal transduction. The SH2 domain of GRB2 binds to tyrosine phosphorylated proteins such as EGFR, IRS-1, Shc and Gab1. The SH3 domain of GRB2 associates with Sos, which stimulates the GTP binding activity of Ras, leading to the activation of the MAP kinase and other signaling pathways. Phosphorylation of tyrosine209 of GRB2 by Bcr-Abl and EGFR abolishes its association with Sos and negatively regulates downstream signaling./p
pbSPECIFICITY: /bThe antibody recognizes 25 kDa GRB2 from samples of human, mouse, and rat origins./p
pbAPPLICATION: /bThe antibody has been used in Western blotting (1 ug/ml). Based on customer?s feedback, the antibody can also be used in immunoprecipitation (10-20 ug/ml), and immunohistochemistry (15-25 ug/ml). However, the optimal concentrations should be determined individually. USAGE:/p
pbFor Research Use Only. Not to be used in human. /b/p