Granzyme B Inhibitor Screening Kit (Fluorometric)

Alternate Name : Granzyme-2 Inhibitor Screening Kit

Tag Line :

Simple Assay, HTS

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence ( Ex/Em = 380/500 nm )
• Applications- Screening Granzyme B inhibitors, characterize/Study Granzyme B inhibitors.

Detection Method:

Features & Benefits:

• Simple, sensitive and high-throughput
• Rapid and convenient
• Includes Inhibitor control, Ac-IEPD-CHO.

Description:

Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In BioVision’s Granzyme B Inhibitor Screening Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. In the presence of potent Granzyme B Inhibitor, the hydrolyzation of substrate will be impeded. The Kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput screening of Granzyme B inhibitors or characterize/study Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors.