Granzyme B Activity Fluorometric Assay Kit

Alternate Name : Granzyme-2 Activity Assay Kit

Tag Line :

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence ( Ex/Em = 380/500 nm )
• Applications- Measurement of Granzyme B activity in various biological samples or purified Granzyme B activity, Screening Granzyme B inhibitors, characterize/Study Granzyme B inhibitors.

Detection Method:

Features & Benefits:

• Simple and Sensitive
• Rapid and convenient
• Kit contains all necessary reagents for measuring Granzyme B Activity.

Description:

Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell. In BioVision’s Granzyme B Activity Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. This Assay Kit is rapid, reliable and sensitive and can detect Granzyme B activity in various biological samples.