Goat anti-UBE2C / UBCH10 Antibody

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

UBE2C; UBCH10; dJ447F3.2; ubiquitin-conjugating enzyme E2C; ubiquitin-protein ligase C; ubiquitin carrier protein E2-C; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; RP3-447F3.1

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Western Blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.

Peptide ELISA: antibody detection limit dilution 1:32000.

 

Background_Info :

 

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(AB only, monoclonals) :

 

Datasheet_Link :

 

Description (Long Description) : 

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit (Ref. Uniprot)

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

 

Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-QETYSKQVTSQEP

 

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