Goat anti-SHH Antibody

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

SHH; sonic hedgehog homolog (Drosophila); HGNC:10848; HHG1; HLP3; HPE3; SMMCI ; sonic hedgehog

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Western Blot: Approx 48kDa band observed in Human Liver lysates (calculated MW of 49.6kDa according to NP_000184.1 Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for this particular batch.Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Peptide ELISA: antibody detection limit dilution 1:32000.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link :

 

Description (Long Description) : 

Sonic hedgehog protein: The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Once cleaved, ShhC is degraded in the endoplasmic reticulum

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

 

Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-DSEALHPLGMAVK

 

Kit_Components :

 

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