Goat anti-PPP1R15A / GADD34 Antibody

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

PPP1R15A; GADD34; protein phosphatase 1, regulatory (inhibitor) subunit 15A; growth arrest and DNA-damage-inducible 34; protein phosphatase 1, regulatory subunit 15A

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Western Blot: Approx 75kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: KLY (Kelly Human neuroblastoma) cell lysate.Immunohistochemistry: In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration: 2-4µg/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Peptide ELISA: antibody detection limit dilution 1:128000.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link :

 

Description (Long Description) : 

Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters (Uniprot).

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

 

Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-AAALDLSGRRG

 

Kit_Components :

 

MSDS (File name) :