Goat anti-OGT Antibody
Antibodies
- 貨號 Cat. No.
- G-1886-100
- 規格 Size
- 100 μg
- 品牌 Brand
- Biosensis
- Application 應用
- Pep-ELISA, WB, IHC, IF, FC
- Reactivity
- Dog and Cow 、 Expected to react with Mouse 、 Human and Rat
- Host
- Goat
- Clonality
- Polyclonal
Description
ZZZZ_Status (Active, Disc) : Active
AA_Supplier Name : Biosensis
Alternative_Name (Other Names) :
OGT; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); FLJ23071; HRNT1; MGC22921; O-GLCNAC ; O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
Antibody_Isotype
(Antibody only) :
Application_Details :
Western Blot: Approx 110kDa band observed in Rat Pancreas lysates calculated MW of 116kDa according to NP_858058.2). An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.05-0.2µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa, U2OS and Glioblastoma U251 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
Background_Info :
Clone_Name
(AB only, monoclonals) :
Datasheet_Link :
Description (Long Description) :
Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1 and can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (Uniprot)..
Expiry_Date :
12 months after purchase if unopened.
Format (Form) :
Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-YEHPKDLKLSDGR
Kit_Components :
MSDS (File name) :
