Goat anti-KIT / CD117 Antibody

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; C-Kit; CD117; PBT; SCFR; mast/stem cell growth factor receptor Kit; p145 c-kit; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; soluble KIT varian

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Western Blot: Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 10µg/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Peptide ELISA: antibody detection limit dilution 1:128000.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link :

 

Description (Long Description) : 

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis.

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

 

Immunogen (Antigen) :A synthetic peptide consisting of amino acids, KRDSFICSKQEDH

 

Kit_Components :

 

MSDS (File name) :