Goat anti-KCNN4 / KCa3.1 Antibody

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

KCNN4; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4; IK1; IKCA1; KCA4; KCa3.1; SK4; hIKCa1; hKCa4; hSK4; intermediate conductance calcium-activated potassium channel protein 1; putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Western Blot: Approx 50kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3 (calculated MW of 47.7kDa, according to Human NP_002241.1 and Mouse NP_032459.3).An additional band of unknown identity was also consistently observed at 35kDa in Cerebellum. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Adrenal Gland lysate.Immunohistochemistry: Paraffin embedded Human Adrenal Gland, Kidney and Liver. Recommended concentration: 3.75µg/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Peptide ELISA: antibody detection limit dilution 1:16000.

 

Background_Info :

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link :

 

Description (Long Description) : 

Forms a voltage-independent potassium channel that is activated by intracellular calcium (PubMed:26148990). Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells (PubMed:17157250, PubMed:18796614). Plays a role in the late stages of EGF-induced macropinocytosis (PubMed:24591580).

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

 

Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-ERQAVNATGHLSD

 

Kit_Components :

 

MSDS (File name) :