Goat anti-Dynactin Antibody

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

DCTN1; dynactin 1 (p150, glued homolog, Drosophila); P135; DP-150; DAP-150; p150-glued; p150, Glued (Drosophila) homolog; 150 kDa dynein-associated polypeptide; dynactin 1 (p150, Glued (Drosophila) homolog); HMN7B; OTTHUMP00000202491; dynactin 1; p150, Glued homolog

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Western Blot: Approx 150kDa band observed in Human Cerebellum lysates (calculated MW of 142kDa according to NP_004073.2). Additional bands between approx. 40-70kDa were consistently observed, and all were successfully blocked by incubation with the immunizing peptide. Recommended concentration 1-2µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4µg/ml. A customer found positive results in IHC on Human HeLa cells.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.
Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells).

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Peptide ELISA: antibody detection limit dilution 1:128000.

 

Background_Info :

 

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(AB only, monoclonals) :

 

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Description (Long Description) : 

Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein (Uniprot).

 

Expiry_Date : 

12 months after purchase if unopened.

 

Format (Form) : 

Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.

 

Immunogen (Antigen) :A synthetic peptide consisting of amino acids, C-QEQLHQLHSRLIS

 

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