GNAI1 Antibody (LS-B2546)

貨號 Cat. No.: LS-B2546
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2546
Antibody Validation:	This antibody replaces catalog number LS-C36537. It has been validated for use in the following assays: IHC-P.
Antibody Target:	GNAI1
Synonyms:	GNAI1 Antibody, Gi Antibody, guanine nucleotide-binding protein g(i), alpha-1 subunit Antibody
Host:	GNAI1 antibody was produced in Mouse
Antigen Species:	GNAI1 antibody was raised against Rat
Clonality:	Monoclonal
Isotype:	IgG2b
Antigen Type:	Protein fraction
Immunogen:	GNAI1 antibody was raised against partially purified Gai1 protein from rat brain (39-42kD). Cellular Localization: Cell membrane.
Antibody Specificity:	Recognizes Rat Gai1. Does not cross-react with Transducin, Gao, Gai2, Gai3, or Gas. ADP-ribosylation of ai1 proteins does not alter the reactivity. Species cross-reactivity: mouse, bovine, guinea pig and human.
Predicted Species Reactivity:	Bovine, Guinea pig, Human, Mouse
Purification:	Protein A Column
Presentation:	PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
Recommended Storage:	Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2546 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2546 was determined to be 1:50. Positive control: IMR-5 cells; brain.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:50), Western blot (WB) (Optimal dilution to be determined by the researcher)
Size:	50 µl