GM1 Ab ELISA

pGM1 Ab ELISAbr /
img alt=For Research Use Only align=absMiddle src=http://www.alpco.com/img/ruo.gif / For Research Use Only/p
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div class=prod_specbFormat:/b/div
div class=prod_spec_infoELISA / EIA/div
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div class=prod_specSample Type:/div
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div class=prod_specbSample Size:/b/div
div class=prod_spec_info20 micro;l/div
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div class=prod_specbSize:/b/div
div class=prod_spec_info96 wells/div
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div class=prod_specbRange:/b/div
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div class=prod_specbSensitivity:/b/div
div class=prod_spec_infoSee Package Insert/div
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div class=prod_specbIncubation Time:/b/div
div class=prod_spec_info4 hour(s) 30 min(s)/div
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pbSummary:/b/p
BUuml;HLMANN anti-GM1 ELISA is based on the enzymeimmunometric assay technique. GM1 has been coated onto the wells of the microtiter plate (Figure 1). Calibrator, controls and patient sera are incubated for two hours in the microtiter wells and anti-GM1 autoantibodies (Ab) present in the sample bind to the immobilized GM1. After washing off unbound substances, horseradish-peroxidase (HRP) labeled antibodies against human IgG and / or IgM are added to the wells and incubated for another two hours. Following a second washing step in which unbound antibody-enzyme reagent is removed, a substrate solution containing tetramethylbenzidine (TMB) is added to the wells. A blue color develops in proportion to the amount of anti-GM1 autoantibodies bound to the microplate in the initial step. Color development is stopped by adding an acidic stop solution (dilute sulfuric acid) which turns the blue solution into yellow. The intensity of the color is measured at 450 nm. The measured absorbance is proportional to the titer of antiganglioside antibodies present in a given sample. The titers of anti-ganglioside autoantibodies are expressed as % ratios of the calibrator and can be assigned to titer categories./div
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