GM-CSF Protein, Mouse, Recombinant

Purity

> 95 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Activity

Measured in a cell proliferation assay using FDC-P1 cells.

The ED50 for this effect is typically 0.012-0.047 ng/mL.

Protein Construction

A DNA sequence encoding the mouse CSF2 (NP_034099.2) (Met1-Lys141) was expressed.

NP#

Expressed Host

HEK293 Cells

Species

Mouse

Predicted N Terminal

Ala 18

Molecule Mass

The recombinant mouse CSF2 consists of 124 amino acids and predicts a molecular mass of 14.1 KDa. It migrates as an approximately 20.7 KDa band in SDS-PAGE under reducing conditions.

Formulation

Lyophilized from sterile PBS, pH 7.4.

1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

2. Please contact us for any concerns or special requirements.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Stability & Storage

Samples are stable for up to twelve months from date of receipt at -70℃

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.