Glycerol-3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit
Kits
- 貨號 Cat. No.
- K640-100
- 規格 Size
- K640-100
- Sample Type
- • Animal tissues - Liver 、 adipocytes 、 brain 、 etc. • Cell culture: Preadipocytes 、 etc.) 、 Heart 、 kidney
Description
Alternate Name : GPDH Activity Colorimetric Assay Kit
Tag Line :
Highly-sensitive assay to measure GPDH activity
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (450 nm)
• Applications:
-Measurement of Glycerol-3-Phosphate Dehydrogenase activity in various tissues/cells
-Analysis of fatty acid metabolism and cell signaling
-Screening anti-obesity drugs
Detection Method:
Features & Benefits:
• Simple, sensitive, and rapid.
• The assay can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.
Description:
Glycerol-3-Phosphate Dehydrogenase (EC 1.1.1.8) is an important enzyme for lipid metabolism. It catalyzes the reversible conversion between dihydroxyacetone phosphate and glycerol-3-phosphate. GPDH plays multiple functions inside cells; it links carbohydrate and lipid metabolism, and provides electrons through the Glycerol-3-Phosphate Shuttle. When progenitor adipocytes differentiate into mature adipocytes, GPDH activity increases significantly. Analysis of glycerol-3-phosphate dehydrogenase activity is crucial for the study of fatty acid metabolic pathways. In BioVision’s Glycerol-3-Phosphate Dehydrogenase Activity Assay kit, Glycerol-3-phosphate dehydrogenase forms an intermediate, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect Glycerol-3-Phosphate dehydrogenase activity less than 1 mU/well.