Kits
Glutathion-S-Transferase (GST) activity, colorimetric
- 貨號 Cat. No.
- K 2631
- 規格 Size
- 100 Tests
- 品牌 Brand
- IDS
Description
| Article number: | K 2631 |
| Method: | colorimetric |
| Tests: | 100 Tests |
| Matrix: | EDTA-whole blood, biological liquids |
| Volume: | 100 µl |
| Incubation time: | 5 min |
| The Glutathione S-Transferase (GST) Assay Kit is intended for the measurement of total GST activity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in erythrocyte lysates. (For research only.) Glutathione-S-transferases (GSTs) are a group of enzymes that are important in the detoxication of many different xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity was found to be present in plants, insects, yeast, bacteria, and in most mammalian tissues, especially in the liver, which plays a key role in detoxification. There are several classes of GST isoenzymes that differ in their specificity toward xenobiotic or endogenous substrates. The Glutathione S-Transferase (GST) Assay Kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB) which is suitable for the broadest range of GST isoenzymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm. |