Glucose Uptake Fluorometric Assay Kit

Alternate Name :

Tag Line :

Non-radioactive, Sensitive Assay

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

• Detection method- Fluorescence (Ex/Em = 535/587 nm)
• Species reactivity- Mammalian
• Applications- This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.

Detection Method:

Features & Benefits:

• Simple procedure
• Fast and convenient
• The assay is easy, non-radioactive, and highly sensitive

Description:

Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to BioVision’s PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.