Glucose Uptake Colorimetric Assay Kit
Kits
- 貨號 Cat. No.
- K676-100
- 規格 Size
- K676-100
- Sample Type
- Living cells (suspension and adherent)
Description
Alternate Name :
Tag Line :
Non-radioactive, Highly Sensitive Assay
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (412 nm)
• Species reactivity- Mammalian
• Application- This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.
Detection Method:
Features & Benefits:
• Simple procedure
• Fast and convenient
• The assay is easy, non-radioactive, and highly sensitive
Description:
Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well.