Glucose and Sucrose Colorimetric/Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K616-100
- 規格 Size
- K616-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 monitoring glucose level during fermentation and glucose feeding in protein expression processes 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method-Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
Detection Method:
Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit provides all necessary buffers and reagents for a accurate assay of glucose and sucrose individually in various biological samples
Description:
Glucose (C₆H₁₂O₆; FW: 180.16) and sucrose (C₁₂H₂₂O₁₁; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase. BioVision’s Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (O.D. 570 nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.