Ghrelin, Acylated ELISA

pGhrelin, Acylated ELISA/p
pimg alt=For Research Use Only align=absMiddle src=http://www.alpco.com/img/ruo.gif / For Research Use Only/p
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div class=prod_specbFormat:/b/div
div class=prod_spec_infoELISA / EIA/div
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div class=prod_specSample Type:/div
div class=prod_spec_infoPlasma/div
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div class=prod_specbSample Size:/b/div
div class=prod_spec_info100 micro;l/div
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div class=prod_specbSize:/b/div
div class=prod_spec_info96 Wells/div
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div class=prod_specbRange:/b/div
div class=prod_spec_info1.95 - 250 pg/ml/div
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div class=prod_specbSensitivity:/b/div
div class=prod_spec_info0.8 pg/ml/div
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div class=prod_specbIncubation Time:/b/div
div class=prod_spec_info3 Hour/div
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pbSummary:/b/p
Ghrelin is present in the peripheral circulation in two forms: acylated and non-acylated. This ELISA specifically measures the acylated form of ghrelin. This ELISA is based on a double-antibody sandwich technique; the Wells of the plate supplied with the kit are coated with a monoclonal antibody specific to the C-terminal part of ghrelin. This antibody will bind to any ghrelin introduced into the wells (standard or sample). The AChE - Fab` conjugate which recognizes the N-terminal part of acylated ghrelin is also added to the wells. This allows the two antibodies to form a sandwich by binding on different parts of the human acylated ghrelin. Each kit contains ghrelin (acylated) AChE-Fab` conjugate, human acylated ghrelin standard, human acylated ghrelin quality control, Ellmanrsquo;s reagent, EIA buffer, wash buffer, Tween 20, plates pre-coated with anti-ghrelin mouse monoclonal antibody, and complete instructions./div
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