GFAP Antibody (LS-B2801)

貨號 Cat. No.: LS-B2801
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2801
Antibody Validation:	This antibody replaces catalog number LS-C7074. It has been validated for use in the following assays: IHC-P.
Antibody Target:	GFAP
Synonyms:	GFAP Antibody, FLJ45472 Antibody, gfap Antibody, glial fibrillary acidic protein Antibody
Host:	GFAP antibody was produced in Mouse
Antigen Species:	GFAP antibody was raised against Porcine
Clonality:	Monoclonal
Isotype:	IgG1
Immunogen:	GFAP antibody was raised against porcine spinal cord. Cellular Localization: Cytoplasmic.
Antibody Specificity:	Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Species cross-reactivity: human, rat and chicken.
Predicted Species Reactivity:	Chicken, Human, Porcine, Rat
Purification:	Protein G Column
Presentation:	PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, 40% glycerol.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2801 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2801 was determined to be 1-2 ug/ml. Immunoprecipitation (Native and denatured): 2 ug/mg protein lysate (Use Protein G). Western Blot: 1-2 ug/ml for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1 - 2 µg/ml), Western blot (WB) (1 - 2 µg/ml), Immunoprecipitation (IP) (2 µg/mg lysate), Immunofluorescence (IF) (Optimal dilution to be determined by the researcher)
Size:	50 µl