Genistein
- 貨號 Cat. No.
- G-300
- 規格 Size
- 10 x 1 mg 10mg 5x10mg 25mg 5x25mg 100mg
Description
Genistein
A Tyrosine Kinase Inhibitor
| Product#: | G-300 |
| Sizes: |
| 10 x 1 mg |
| 10 mg |
| 5 x 10 mg |
| 25 mg |
| 5 x 25 mg |
| 100 mg |
| M.W.: | 270.2 Daltons |
| CAS No.: | [446-72-0] |
| Structure: |
| . |
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| Purity: | >99% |
| Bioassay: |
| The biological activity was tested to confirm the ability of Genistein to inhibit tyrosine kinase activity in Hela cells. |
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| Hela cells were grown to 70% confluence then the cells serum starved for 18 h. The cells were preincubated for 30 min in presence or absence of 500 nM Genistein and then simulated for 2 h with 20% fetal calf serum or culture medium. The cell extracts were blotted and probed with p-Tyr specific antibodies. |
| References: |
1. | Polkowski, K. and Mazurek, A.P. (2000) Acta Pol. Pharm. 57, 135. Review. |
2. | Sarkar, F.H. and Li, Y. (2002) Cancer and Metastasis Reviews 21, 265. Review. |
3. | Salti, G.I (2000) Eur. J. Cancer. 36, 796. |
4. | Spinozzi, F. et al. (1994) Leuk. Res. 18, 431. |
5. | Constantinou, A. et al. (2003) Cancer Sci. 94, 215. |
6. | Li, Y. and Sarkar, F.H. (2002) Cancer Lett. 186, 157. |
7. | Huang, R.Q. et al. (1999) Brain Res. Mol. Brain Res. 67, 177. |
8. | Constantinou, A. and Huberman, E. (1995) Proc. Soc. Exp. Biol. Med. 208, 109. Review. |
9. | Khoshyomn, S. et al. (2002) J. Neurooncol 57, 193. |
10. | Tamura, S. et al. (2003) Pigment Cell Res. 16, 470. |
An isoflavonoid derived from soy products.1 Genistein inhibits protein tyrosine kinases, including the EGF receptor kinase (IC50 = 2.6 uM). The inhibition is competitive with respect to ATP and non-competitive with respect to the phosphate acceptor.1,2 It has a trivial effect on the activity of PKA and PKC (IC50 > 350 uM).1,2 In addition, Genistein inhibits Topoisomerase II activity,3 cause cell cycle arrest and apoptosis in Jurkat T-leukemia cells.4 It down regulates the transcription of genes involved in angiogenesis,5,6 and inhibit the action of GABA on recombinant GABA-A receptors.7 Genistein also inhibits tumor cell proliferation and induces tumor cell differentiation. 8-10