GCLC Antibody (LS-B2328)

貨號 Cat. No.: LS-B2328
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2328
Antibody Validation:	This antibody replaces catalog number LS-C15310. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Glutamate-cysteine Ligase, Catalytic Subunit (GCLC)
Synonyms:	GCLC
Host:	GCLC antibody was produced in Rabbit
Antigen Species:	GCLC antibody was raised against Rat
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide
Immunogen:	GCLC antibody was raised against a synthetic peptide derived from corresponding to aa295-313 of rat GCLC (heavy subunit of GCS).
Antibody Specificity:	Recognizes the heavy subunit of rat GCS. Species cross-reactivity: human, monkey and mouse. Cross-reacts weakly with bovine.
Antigen Modification:	aa295-313
Predicted Species Reactivity:	Bovine, Human, Monkey, Mouse
Purification:	Protein A Column
Presentation:	PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2328 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2328 was determined to be 5 ug/ml. Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 2.5-5 ug/ml for 2hrs at RT. Immunoprecipitation: 10 ug/mg protein lysate. Denatured verified. Use Protein A. Positive control: Jurkat, MAD109, PC12 cells.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), Immunoprecipitation (IP), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µl
Concentration:	1 mg/ml