GCK (Glucokinase) Antibody

Antibody Target :GCK (Glucokinase)

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide selected from the N-terminal region of human GCK

Accession#: P35557

Gene ID: 2645

Appearance: Colorless liquid

Concentration: 0.25 mg/ml

Formulation: 100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Purification: Affinity purified

Species Reactivity: hu/ms

Application: ELISA/WB/IHC

Application & Usage: The antibody can be used for ELISA (0.25 µg/ml), Western Blotting (0.5 – 2.5 µg/ml) and Immunohistochemistry (2.5 – 5.0 µg/ml).

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of the gene for GCK results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in the gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), also called maturity onset diabetes of the young, type 2 (MODY2); mutations have also been associated with persistent hyperinsulinemic hypoglycemia of infancy (PHHI).