GATA4 Antibody (LS-B2269)

貨號 Cat. No.: LS-B2269
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2269
Antibody Validation:	This antibody replaces catalog number LS-C7056. It has been validated for use in the following assays: IHC-P.
Antibody Target:	GATA Binding Protein 4 (GATA4)
Synonyms:	GATA4, GATA-binding protein 4, transcription factor gata-4
Family:	Transcription factor
Subfamily:	not assigned-Transcription factor
Host:	GATA4 antibody was produced in Rabbit
Antigen Species:	GATA4 antibody was raised against Human
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide
Immunogen:	GATA4 antibody was raised against synthetic phosphopeptide derived from a region of human GATA-4 that contains serine 105. The sequence is conserved in mouse, rat and chicken.
Antibody Specificity:	Recognizes human GATA-4. Species sequence homology: mouse, rat and chicken.
Antigen Modification:	pSer105
Predicted Species Reactivity:	Chicken, Mouse, Rat
Purification:	Immunoaffinity Chromatography
Presentation:	PBS (without Mg2+, Ca2+), pH 7.3, 1.0 mg/mL BSA (IgG, protease free), 0.05% sodium azide.
Recommended Storage:	Long term: -20°C; Short term: +4°C
Antibody Usage Summary:	Immunohistochemistry: LS-B2269 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2269 was determined to be 5 ug/ml. Positive control: Phenylephrine (PE)-stimulated rat cardiomyocytes, transfected with mouse GATA-4.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µl
Concentration:	0.35 mg/ml