Proteins and Peptides
GAP43 Antibody (LS-B2157)
- 貨號 Cat. No.
- LS-B2157
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2157 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C7321. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Growth Associated Protein 43 (GAP43) |
| Synonyms: | GAP43, gap-43, gap43, neuromodulin |
| Family: | Neuromodulin |
| Subfamily: | Not assigned-Other |
| Host: | GAP43 antibody was produced in Mouse |
| Antigen Species: | GAP43 antibody was raised against Rat |
| Clonality: | Monoclonal |
| Isotype: | IgG2a,k |
| Antigen Type: | Purified protein |
| Immunogen: | GAP43 antibody was raised against purified, full length GAP-43 protein (both phosphorylated & unphosphorylated forms). |
| Antibody Specificity: | Specific for the detection of GAP-43 and reacts with both phosphorylated and unphosphorylated forms of the GAP-43 protein. Reactivity with GAP-43 has been confirmed by Western blotting using total cell lysates derived from mouse and rat brain. Species cross-reactivity: Chicken, human, mouse, rabbit, rat and snake. |
| Predicted Species Reactivity: | Chicken, Human, Mouse, Rabbit |
| Purification: | Protein A Column |
| Presentation: | PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%. |
| Recommended Storage: | Long term: Add glycerol (40-50%) -20°C; Short term: +4°C |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2157 was determined to be 5 ug/ml. Positive control: Cell lysates derived from mouse and rat brain. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr) (5 µg/ml), Western blot (WB) (1 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
| Concentration: | 0.25 mg/ml |


