GAP43 Antibody (LS-B2157)

貨號 Cat. No.
LS-B2157
規格 Size
50 µg
品牌 Brand
LSBio

Description

























































































Antibody Catalog ID: LS-B2157
Antibody Validation: This antibody replaces catalog number LS-C7321. It has been validated for use in the following assays: IHC-P.
Antibody Target: Growth Associated Protein 43 (GAP43)
Synonyms: GAP43, gap-43, gap43, neuromodulin
Family: Neuromodulin
Subfamily: Not assigned-Other
Host: GAP43 antibody was produced in Mouse
Antigen Species: GAP43 antibody was raised against Rat
Clonality: Monoclonal
Isotype: IgG2a,k
Antigen Type: Purified protein
Immunogen: GAP43 antibody was raised against purified, full length GAP-43 protein (both phosphorylated & unphosphorylated forms).
Antibody Specificity: Specific for the detection of GAP-43 and reacts with both phosphorylated and unphosphorylated forms of the GAP-43 protein. Reactivity with GAP-43 has been confirmed by Western blotting using total cell lysates derived from mouse and rat brain. Species cross-reactivity: Chicken, human, mouse, rabbit, rat and snake.
Predicted Species Reactivity: Chicken, Human, Mouse, Rabbit
Purification: Protein A Column
Presentation: PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Recommended Storage: Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Antibody Usage Summary: Immunohistochemistry: LS-B2157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2157 was determined to be 5 ug/ml. Positive control: Cell lysates derived from mouse and rat brain.
Antibody Uses: Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Immunohistochemistry - Frozen (IHC-Fr) (5 µg/ml), Western blot (WB) (1 µg/ml), ELISA (0.1 - 1 µg/ml) (Optimal dilution to be determined by the researcher)
Size: 50 µg
Concentration: 0.25 mg/ml

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