Galactose and Lactose Colorimetric/Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K617-100
- 規格 Size
- K617-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue culture supernatants 、 monitoring levels during fermentation and feeding in protein expression processes 、 Plasma and Serum 、 urine
Description
Alternate Name :
Tag Line :
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ 1 hour
• Fast and convenient
• Kit provides all necessary buffers and reagents for a accurate assay of galactose and lactose individually in various biological samples
Description:
Galactose (C₆H₁₂O₆ FW: 180.16) and lactose (C₁₂H₂₂O₁₁ FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. BioVision’s Galactose / Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (O.D 570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose – Free Galactose.