GADD 153 (K121) Antibody

貨號 Cat. No.

BS1136

規格 Size

100μg

品牌 Brand

Bioworld

Description

stock

Host: Rabbit

Reactivity: Human,Mouse,Rat

Product

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

GADD153/DDIT3 (K121) antibody detects endogenous levels of GADD153/DDIT3 protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Western Blot(WB)

Western blot (WB) analysis of GADD153/DDIT3 (K121) antibody in extracts from LOVO treated with serum 10% 15' cells.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC) analyzes of GADD153/DDIT3 (K121) antibody in paraffin-embedded human breast carcinoma tissue.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

GADD 153 has been described as a growth arrest and DNA damageinducible gene that encodes a C/EBP-related nuclear protein. This protein has also been designated C/EBP-homologous protein (CHOP-10). GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations. GADD 153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from “classical” C/EBP binding sites, recognizing instead unique “nonclassical” sites. Thus GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells, similar to the regulatory function of Id on the activity of Myo D and Myo D-related proteins involved in the development of muscle cells.

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