G26.4.1C3/86

G26.4.1C3/86 Whole Cell Lysate  

Catalog No: GRL-HLC033

Size: 200 μg 

Cell Line: G26.4.1C3/86 (Lymphoblast hybridoma)

Growth Media:

RPMI 1640 medium with 2mM L-glutamine+10mM HEPES+1mM sodium pyruvate+4.5g/L glucose+1.5g/L sodium bicarbonate+15% fetal bovine serum

Cell Lysate Preparation:  

。Prepare cell lysate by homogenization in ice-cold modified RIPA Lysis Buffer. 

。After centrifugation, determine the protein concentration of the cell lysate. 

。Boil the lysate for 5 minutes in 1X SDS sample buffer. 

。The lysate is ready to load onto SDS-PAGE for Western blotting. (20~50 μg per lane is recommended for mini gel.) 

Reagents: 

RIPA buffer: 150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.

SDS sample buffer: 50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol. 

Application:

G26.4.1C3/86 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 μg~50 μg per lane is recommended for mini gel. 

Storage: 

It is supplied as whole cell lysate at 100 μg at 2 mg/ml in 1 x SDS sample buffer containing 5% beta-mercaptoethanol. Store at -20°C for three months or at –70°C for one year.