FractionPREP™ Cell Fractionation Kit

Kits

貨號 Cat. No.
K270-50
規格 Size
K270-50
Sample Type
Cell cultures (adherent and suspension) 、 fresh and frozen tissues

Description




Alternate Name :


 

Tag Line :



 

Storage Conditions: -20°C


 

Shelf life: 12 months


 

Summary :


• Species reactivity- Mammalian
• Applications- 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.


 

Detection Method:


 

Features & Benefits:


• Simple procedure; takes less than 1 hour
• Fast and convenient
• The kit is designed for serial sample preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions, from one sample.


 

Description:


This FractionPREP™ Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.


 





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