Fraction II, rabbit reticulocyte
- 貨號 Cat. No.
- F-360
- 規格 Size
- 2.5 mg 5 mg
- 品牌 Brand
- Boston Biochem
Description
Fraction II, rabbit reticulocyte
rabbit reticulocyte
Product Information
2.5 mg 5 mg
Use & Storage
Ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radio-labeled or immuno-detectable substrates. The inclusion of an isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the efficient accumulation of ubiquitin protein conjugates. Typical assay concentrations for FII are 0.5 - 4 mg/ml.
Store at -80°C. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.
Catalog #: F-360
Fraction II, rabbit reticulocyte
Product Source:
rabbit reticulocyte
Also known as: FII
Protein fraction from a cell extract that binds to an anion exchange resin. Essentially ubiquitin-free and ATP-free, FII contains E1, most E2s, some E3s, UCHs, and the proteasome (20S and 26S).
Product Information
Quantity:
2.5 mg 5 mg
Stock:
10.0 mg/ml in 25 mM HEPES pH 7.6.
Actual protein concentration will vary with specific lot number.
Use & Storage
Use:
Ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radio-labeled or immuno-detectable substrates. The inclusion of an isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the efficient accumulation of ubiquitin protein conjugates. Typical assay concentrations for FII are 0.5 - 4 mg/ml.
Storage:
Store at -80°C. For best results, aliquot and freeze after first set of experiments to avoid multiple freeze/thaw cycles.