Formate Colorimetric Assay Kit

Kits

貨號 Cat. No.
K653-100
規格 Size
K653-100
Sample Type
as well as many other biological fluids 、 Cell and tissue culture lysates 、 Plasma and Serum 、 urine

Description




Alternate Name :


 

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Storage Conditions: -20°C


 

Shelf life: 12 months


 

Summary :


• Detection method- Absorbance (450 nm)
• Species reactivity- Mammalian
• Applications- The assay can detect formate in the range from 0.5 to 10 nmols/well.


 

Detection Method:


 

Features & Benefits:


• Simple procedure; takes ~60 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of formate levels in a number of sample types


 

Description:


Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate. In the Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.


 





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