Follistatin, human recombinant

Alternate Names / Synonyms: FST, FS, Activin-binding protein

Gene Symbol: FST

Accession #: P19883

Gene ID: 10468

Gene Source: Human

Recombinant Host: E. coli

Appearance: Lyophilized protein

Physical Form: Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 75 mM NaCl

Molecular Weight: 31.5 kDa

Purity by SDS-PAGE: ≥95%

Purity by HPLC: N/A

Endotoxin Level: <0.1 ng/μg

Biological Activity: Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected EDâ‚…â‚€ is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.

Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Storage Temp.: -20°C

Shipping: Gel pack

Background Information: Follistatin belongs to a group of structurally-diverse diffusible proteins that binds to TGF-β ligands and inhibit their activity by hindering access to signaling receptors. This naturally occurring antagonist binds to Activin, BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-β1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. Recombinant human Follistatin is a 31.5 kDa protein containing 288 amino acids including 36 cysteine residues.

Handling: Centrifuge the vial prior to opening.