Folicle Stimulating Hormone (FSH) (Rat) ELISA

pFolicle Stimulating Hormone br /
(FSH) (Rat) ELISAbr /
nbsp;/p
pimg alt=For Research Use Only src=http://www.alpco.com/img/ruo.gif align=absMiddle / For Research Use Only/p
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div class=prod_specbFormat:/b/div
div class=prod_spec_infoELISA / EIA/div
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div class=prod_specSample Type:/div
div class=prod_spec_infoserum, plasma/div
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div class=prod_specbSample Size:/b/div
div class=prod_spec_info25 micro;l/div
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div class=prod_specbSize:/b/div
div class=prod_spec_info96 wells/div
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div class=prod_spec_box
div class=prod_specbRange:/b/div
div class=prod_spec_info0.78 - 50 ng/ml/div
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div class=prod_specbSensitivity:/b/div
div class=prod_spec_info0.2 ng/ml/div
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div class=prod_specbIncubation Time:/b/div
div class=prod_spec_info3 Hour 30 Min/div
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pbSummary:/b/p
The ELISA technique uses antibodies with high affinity and specificity for two different epitopes on rat FSH. A first mouse anti rFSH monoclonal antibody bound to a polystyrene well will capture the rFSH of the sample in the presence of a second horseradish peroxidase conjugated mouse anti-rFSH monoclonal antibody. Following the incubation and the one step formation of the solid phasendash;rFSH-conjugated monoclonal antibody sandwich, the well is washed to remove excess unbound conjugated antibody. Then the chromogen/substrate is added, which turns from pink to blue proportionally to the rat follitropin (rFSH) concentration in the sample. Addition of the stop solution turns the color to yellow. The intensity of the yellow color is measured using a spectrophotometer with a 450 nm filter. Sample concentrations are read from a calibration curve and the results are expressed in ng/ml./div
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