Fluoro H2O2 - Fluorescent Hydrogen Peroxide/Peroxidase Detection Kit

Key Benefits:

·         Quick 10 minute assay

·         Can monitor multiple time points to follow kinetics.

·         Dual mode, can detect H₂0₂ or peroxidase activity.

·         One-step, no wash assay.

·         Adaptable for High Throughput format

·         Non-destructive cell based assay allows monitoring of additional parameter

·         Applications - Fluorescent Plate Reader

Assay Principle:

The Fluoro H₂0₂ detection kit utilizes a non-fluorescent detection reagent, to detect H₂0₂. H₂0₂ oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.

The detection reagent can be utilized to detect H₂0₂ release from cells or enzyme coupled reactions (1-7)

Kit Contents:

1.       5X Reaction Buffer: 20 ml buffer, pH 7.4

2.       Detection reagent: One vial for 500 assays.

3.       Hydrogen Peroxide: 200µL of a stabilized 3% solution.

4.       Horseradish Peroxidase: 18.9 Units of enzyme