Fluoro EPO - Fluorescent Eosinophil Peroxidase Detection Kit

Eosinophils are involved in initiation and propagation of diverse inflammatory responses of innate and adaptive immunity, particularly against parasitic infection. Eosinophil peroxidase (EPO) is the major cytotoxic agent released by activated eosinophils and uses hydrogen peroxide to generate reactive oxidants from halides and pseudo halide thiocyanate. EPO exhibits antimycobacterial activity and is implicated in tissue damage that occurs in the course of asthma and other diseases. Fluoro EPOä is a sensitive one-step no-wash assay to measure EPO activity. It can establish kinetics by monitoring multiple time points, and is suitable for the high throughput format. Application: Fluorescence microplate reader (excitation at 530-571 nm, emission at 590-600 nm).