Fluorescein Ubiquitin
- 貨號 Cat. No.
- U-590
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
Fluorescein Ubiquitin
human recombinant
Product Information
100 μg
Soluble in aqueous buffer or DMSO up to 20 mg/ml.
8.6 kDa
> 95% by PAGE
This is a lyophilized powder
Use & Storage
Fluorescein-N-term-ubiquitin gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.
Catalog #: U-590
Fluorescein Ubiquitin
Product Source:
human recombinant
Ubiquitin modified with fluorescein via primary amine coupling. This results in multiple fluoresceinated ubiquitin species with modified lysines as well as the N-terminus. Although having a fully functional C-terminus, lysine modification may limit the ability of this reagent to efficiently incorporate into poly-ubiquitin chains.
Product Information
Quantity:
100 μg
Stock:
Soluble in aqueous buffer or DMSO up to 20 mg/ml.
MW:
8.6 kDa
Purity:
> 95% by PAGE
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Fluorescein-N-term-ubiquitin gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.
Storage:
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.