Fluorescein-NEDD8
- 貨號 Cat. No.
- UL-830
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Fluorescein-NEDD8
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 20 mM HEPES pH 8.0. Actual concentration will vary with specific Lot #.
9 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-830
Fluorescein-NEDD8
Product Source:
human recombinant
NEDD8 modified with fluorescein via primary amine coupling is ideal as an alternative to radiolabeled NEDD8. This results in multiple fluoresceinated NEDD8 species with modified lysines as well as the N-terminus. The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the UbcH12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 20 mM HEPES pH 8.0. Actual concentration will vary with specific Lot #.
MW:
9 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Fluorescein-NEDD8 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex494 nm and Em520 nm wavelengths respectively.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.