Fluorescein-N-terminal Ubiquitin
- 貨號 Cat. No.
- U-580
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Fluorescein-N-terminal Ubiquitin
human recombinant
Product Information
50 μg
Soluble in aqueous buffer or DMSO up to 20 mg/ml.
8.6 kDa
> 95% by PAGE
This is a lyophilized powder
Use & Storage
Fluorescein-N-term-ubiquitin gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.
Catalog #: U-580
Fluorescein-N-terminal Ubiquitin
Product Source:
human recombinant
Produced via a proprietary process resulting in a single fluorescein modification exclusively on the N-terminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin chain incorporation. This reagent allows for poly-ubiquitin chain incorporation of fluorescein-N-terminal ubiquitin with higher efficiency and detection sensitivity than traditionally modified ubiquitins.
Product Information
Quantity:
50 μg
Stock:
Soluble in aqueous buffer or DMSO up to 20 mg/ml.
MW:
8.6 kDa
Purity:
> 95% by PAGE
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Fluorescein-N-term-ubiquitin gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.
Storage:
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.