Fluorescein-His6-UFM1
- 貨號 Cat. No.
- UL-521
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Fluorescein-His6-UFM1
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1mM DTT, 10% glycerol. Concentration varies by Lot#.
12.1 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.
Catalog #: UL-521
Fluorescein-His6-UFM1
Product Source:
human recombinant
UFM1 modified with fluorescein via primary amine coupling, resulting in the modification of lysine residues as well as the N-terminus. This labeled Ufm1 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6 tag.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 1mM DTT, 10% glycerol. Concentration varies by Lot#.
MW:
12.1 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Fluorescein-UFM1 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.
Storage:
Store at -80°C once reconstituted. Avoid multiple freeze/thaw cycles.