Fluorescein-His6-Apg8p1 (GABARAP)

貨號 Cat. No.
UL-414
規格 Size
50 μg
品牌 Brand
Boston Biochem

Description

Fluorescein-His6-Apg8p1 (GABARAP)

Catalog #: UL-414




Fluorescein-His6-Apg8p1 (GABARAP)






Product Source: 

human recombinant



Apg8p1 modified with fluorescein via primary amine coupling resulting in the modification of lysine residues as well as the N-terminus. This labeled Apg8p1 allows for direct detection spectophotometrically with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6 tag.


Product Information



Quantity: 

50 μg






Stock: 

X mg/ml (XμM) in 50mM Hepes pH 7.5, 100mM NaCl. Actual concentration varies with lot number.






MW: 

16 kDa






Purity: 

> 95% by SDS-PAGE




 


Use & Storage



Use: 

Fluorescein- His6-Apg8p1 gives a strong signal in the range of 0.1-1 μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex490 nm and Em515 nm wavelengths respectively.






Storage: 

Store at –80°C. Avoid multiple freeze/thaw cycles.



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