Florfenicol ELISA Kit

Alternate Name :

Tag Line :

A Competitive ELISA kit for in vitro quantitative determination of Florfenicol concentrations in Tissue, liver, Egg, Honey, Milk powder, Feed

Storage Conditions: 4°C

Shelf life: 12 months

Summary :

Detection Method: Absorbance (450 nm)

Features & Benefits:

Sensitivity: 0.15 ppb (ng/mL), Sample recovery rate: Tissue, Liver - 85%±20%; Honey- 85%±25%; Feed, Milk - 75%±25%., Cross-reactivity: Florfenicol -100%; Thiamphenicol - 0.1%, Detection range: Tissue, Liver, Honey, Milk - 0.075ppb; Feed, Milk powder - 0.15 ppb

Description:

Florfenicol is a broad-spectrum, primarily bacteriostatic, antibiotic with a range of activity similar to that of chloramphenicol, including many gram-negative and gram-positive organisms. It has a same mechanism of action as chloramphenicol i.e. inhibition of protein synthesis. Signs of Florfenicol toxicity are varied and include diarrhea and hyperbilirubinemia in horses, diarrhea and decreased feed consumption and rumen activity in cattle. Overdose or prolonged florfenicol administration can cause fatal bone marrow suppression. Florfenicol ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Florfenicol. During the reaction, Florfenicol in the samples or standard competes with Florfenicol coated on the plate for binding to the anti-Florfenicol antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Florfenicol. The concentration of Florfenicol in the samples can be calculated by comparing the OD of the samples to the standard curve.