Antibodies
FLI-06
- 貨號 Cat. No.
- S7399
- 規格 Size
- 10mgã€25mg
- 品牌 Brand
- Selleckchem
Description
FLI-06
Catalog No.S7399
FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM.
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Distributor Tel: +886-2-2827-1197jimmy@new.abscience.com.tw
Biological Activity
| Description | FLI-06 is a novel inhibitor of Notch signaling with EC50 of 2.3 μM. | |||||
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| Targets | Notch | |||||
| IC50 | 2.3 μM (EC50) [1] | |||||
| In vitro | In HeLa NotchΔE-eGFP cells, FLI-06 blocks Notch trafficking and processing. FLI-06 changes the maturation pattern of APP and abolishes the shedding of APPs in HEK293 cells stably expressing a mutated APP that yields robust amounts of amyloid β. FLI-06 disrupt the Golgi apparatus, and inhibits general secretion at a step before exit from the endoplasmic reticulum (ER), which is accompanied by a tubule-to-sheet morphological transition of the ER. [1] | |||||
| In vivo | FLI-06 (50 μM) inhibits endogenous Notch signaling in zebrafish embryos. [1] | |||||
| Features | ||||||
Protocol(Only for Reference)
Kinase Assay: [1]
| EC50 determinations | EC50 values of FLI-06 is calculated from serial dilution series ranging from 200 to 0.1 μM. Cells are seeded in 96-well plates at a density of 5,000 cells per well in 100 μL medium. The next day, 100 μL medium containing FLI-06 is added. Cells are incubated for 16 h, fixed and processed for automated microscopy. For the putative GSIs, the enuc intensity is divided by the DAPT control, and for the trafficking inhibitors, the normalized percentage inhibition against DAPT or DMSO controls of log2-transformed nuc-to-enuc ratios are calculated. Relative activity values are read into R, and EC50 estimates are calculated using four-parameter log-logistic fit with the package drc46. |
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Animal Study: [1]
| Animal Models | Zebrafish embryos. |
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| Dosages | 50 μM |
| Administration |
References
Chemical Information
| Molecular Weight (MW) | 438.52 |
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| Formula | C25H30N2O5 |
| CAS No. | 313967-18-9 |
| Storage | 3 years -20℃Powder |
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| 6 months-80℃in DMSO | |
| Syonnyms | N/A |
| Solubility (25°C) * | In vitro | DMSO | 88 mg/mL (200 mM) |
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| Water | <1 mg/mL (<1 mM) | ||
| Ethanol | 8 mg/mL (18 mM) | ||
| * <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. | |||
| Chemical Name | cyclohexyl 2,7,7-trimethyl-4-(4-nitrophenyl)-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate |
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