FLAG-Ubiquitin
- 貨號 Cat. No.
- U-120
- 規格 Size
- 1 mg
- 品牌 Brand
- Boston Biochem
Description
FLAG-Ubiquitin
human recombinant
Product Information
1 mg
9.3 kDa
> 95% by SDS-PAGE
Aqueous solutions up to 10 mg/ml
This is a lyophilized powder
Use & Storage
Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.
Catalog #: U-120
FLAG-Ubiquitin
Product Source:
human recombinant
This fully functional N-terminal FLAG-tagged ubiquitin protein allows for the convenient detection or affinity purification of ubiquitinated proteins in vitro. The epitope tag is a hydrophilic octapeptide (DYKDDDDK) derived from the sequence of the bacteriophage 7 gene-10 product. This tag is specifically recognized by anti-FLAG antibodies M1, M2 or M5. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosomeindependent events such as protein localization, activity and function.
Product Information
Quantity:
1 mg
MW:
9.3 kDa
Purity:
> 95% by SDS-PAGE
Solubility:
Aqueous solutions up to 10 mg/ml
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentration to support in vitro conjugation is 500 μM to 1 mM depending on conditions
Storage:
Lyophilized powder at 4 degrees C. Solubilized stock solution at -20 degrees C. Avoid multiple freeze/thaw cycles.