FLAG-NEDD8 Vinyl Sulfone
- 貨號 Cat. No.
- UL-803
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
FLAG-NEDD8 Vinyl Sulfone
human recombinant
Product Information
25 μg
9.1 kD
>90%
Use & Storage
Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 µM.
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-803
FLAG-NEDD8 Vinyl Sulfone
Product Source:
human recombinant
This N-terminal HA-tagged NEDD8 is a potent, irreversible inhibitor of NEDD8-specific isopeptidases (like NEDP1 or SENP8, Cat # E-800).Useful for inhibiting the hydrolysis of poly-NEDD8 chains on substrate proteins in vitro and thus enhances poly-NEDD8 chain accumulation.The HA peptide sequence (YPYDVPDYA) is derived from the influenza hemagglutinin protein. This epitope allows for the sensitive identification or purification of NEDD8 deconjugating activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (XμM) in 50 mM Hepes pH 7.0, 150 mM sodium acetate, 10 % glycerol. Concentration varies with Lot #.
MW:
9.1 kD
Purity:
>90%
Use & Storage
Use:
Add directly to in vitro assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs in vitro are 1-5 µM.
Storage:
Store stock solution at -80°C. Avoid multiple freeze/thaw cycles.