Fibronectin Antibody (LS-B2318)

貨號 Cat. No.: LS-B2318
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2318
Antibody Validation:	This antibody replaces catalog number LS-C23593. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Fibronectin 1 (FN1)
Synonyms:	FN1, fibronectin
Host:	FN1 antibody was produced in Rabbit
Antigen Species:	FN1 antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Purified protein
Immunogen:	FN1 antibody was raised against fibronectin purified from human plasma.
Antibody Specificity:	Recognizes human fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI collagens or laminin. <1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. Non-specific cross-reactivity of anti-fibronectin antibodies with other human serum proteins or non-fibronectin extracellular matrix proteins is negligible.
Purification:	Affinity Chromatography
Presentation:	BBS, 5 mM EDTA, pH 8.0, 0.01% sodium azide, before the addition of glycerol to 40%
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2318 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2318 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1:2000 - 1:4000), Immunoprecipitation (IP), ELISA (1:2000 - 1:4000) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	0.5 mg/ml